Non-volatile liquids are very hard to separate by normal physical means. For this reason, thin layer chromatography is used. In this action, a very thin sheet of aluminum foil or plastic is used as the separating medium. thin layer chromatography equipment. The film is generally coated with an adsorbent material, which is usually silica, aluminum oxide, or cellulose. Thanks to this adsorbent material, the sample under test gets separated, and the consisting liquids appear as spots on the entire foil sheet.

Thin Layer Chromatography Analysis

At Nishka, we practice different kinds of thin layer chromatography to ensure the results procured have a hundred percent accuracy and precision. thin layer chromatography examples. Our experts spend lots of time in preparing the sample, arranging the setup for the thin layer chromatography analysis and then, proceeding with the separation process. During every examination, our primary concern involves the physical environment, and that is why our experts ensure the experiments are done under controlled situations.

Principle of TLC at Nishka

The TLC principle relies on the affinity of the sample compounds with the stationary phase of the adsorbent material coating the film. Here, once the sample is placed on a sheet at a beginning point, the compounds having a higher affinity to the stationary phase moves slowly, thereby creating a low-velocity mobile phase. As for the compounds having low affinity, gain more mobile phase velocity and spread further.

As the compounds get separated, they form spots along the line markers made on the sheet. After the separation, each component is then studied in different ways to determine their physical behaviors and chemical characteristics. Our experts at Nishka use the standard marker sheet to ensure that the study of the compounds can be done with reference to a set of knowledge. Also, multiple tests are run to ensure the results are the same throughout.

Once the deduction is complete, they collect the results and then move to study the characteristics by implementing other different methods.

Our TLC experts art Nishka

We have a great team of expert professionals who know every single thing associated with thin layer chromatography. The various laboratory apparatus and set-ups used here are of high quality. They are sterilized before being used so that the obtained results are authentic, with no error in them. All our experts are certified and licensed. You don’t have to worry about the results being tarnished or not up to the mark.

Regardless of how complicated the sample compound is, we love the challenge and are always keen on testing new compounds to understand the chemical and physical behaviors. So, if you are involved in any industry where non-volatile liquids are one of the raw ingredients, look further into our TLC services at Nishka.