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High Performance Liquid Chromatography

High Performance Liquid Chromatography (HPLC)

“When minute differences in solubility come into play, it is the High-Performance Liquid Chromatography (HPLC) that comes to the rescue.”

High-Performance Liquid Chromatography (HPLC) is a type of chromatography where pressure is used for the separation of the elements. high performance liquid chromatography process. This is why it was formerly named High-Pressure Liquid Chromatography. The technique is useful for compounds that are thermally labile or are not volatile. Here, the target mixture is passed in the solvent at high pressure via the column that contains the chromatographic materials.

At Nishka, we have numerous HPLCs that are used for the separation of different types of molecules depending on size, polarity, etc. high performance liquid chromatography lab. We offer the testing of a wide array of materials high performance liquid chromatography equipment with the assistance of the High-Performance Liquid Chromatography technique. Our team of expert lab technicians and professionals can handle the various materials, even if they are at trace levels.

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Key properties evaluated using HPLC

At Nishka, the HPLC laboratories are set up following the standard regulation protocols. We conduct the testings under sterile environmental conditions. Moreover, we make sure that there are no trace contaminants to dilute or cause an error in the results. For that, we take several precautionary steps.
Here, firstly, the sample is pushed into the solvent known as the mobile phase. Then, it is put across the high pressure in the stationary phase that contains different packing materials. The sample is moved with the aid of gas flow of either nitrogen or helium.

The main feature of HPLC is that it can separate and identify the components present in the sample. It doesn’t matter if it is in trace amounts, such as the trillionth part. Due to this versatility nature, the technique is used across various industries such as chemical, pharmaceutical, forensics, etc.

Industries Served
  • Aerospace
  • Automotive
  • Biomedical/biotechnology
  • Compound Semiconductor
  • Data Storage
  • Defense
  • Displays
  • Electronics
  • Industrial Products
  • Lighting
  • Pharmaceutical
  • Photonics
  • Polymer
  • Semiconductor
  • Solar Photovoltaics
  • Telecommunications
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However, the retention time of the sample varies based on several factors. Some of them are – analysis of the molecules, the interactivity that takes place at the stationary phase, etc. When the sample moves across the column, the interaction between the two distinct phases does not occur at the same rate. It is because both of these phases have different polarities. Now, the analytes that have less interaction at the stationary phase will come out of the column first, followed by the others.

The equipment pieces that we here at Nishka are of the highest quality. Moreover, we focus on obtaining the latest models to ensure that we can get the best results possible. We have a team of adroit technicians that can deal with the testing with efficiency. Our professionals strive to ensure that there is the least amount of flaws or discrepancies in the result. You can count on us to offer best-quality services.

Nishka Research

NISHKA has been helping customers unlock their full potential and realize their true value for more than 13 years by providing innovative and bespoke solutions to ensure the highest standards.
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Nishka Research Pvt. Ltd.

Regus Business Center,
4th floor, Gumidelli Commercial Complex,
1-10-39 to 44, Old Airport Road, Begumpet,
Hyderabad-500016
India

Phone : +91 40 29303155

Mobile : +91 7842798518

Send Mail : [email protected]
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Aishwarya Katrela
Aishwarya Katrela

Left us a 5 star review

Aishwarya Katrela
April 27, 2022
Tejaswini Kulakarni
December 14, 2021
Keyur Butani
May 10, 2022

Submitted the samples for my phd work . Great work and reports submited with 2 days of time . Also the image clarity and all data are clear .

Chaithanya Reddy
December 30, 2021
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December 14, 2021
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August 22, 2021
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December 15, 2021
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December 14, 2021
Anita Raj Panigrahi
October 20, 2021

Thank you for your services.. Looking forward for more collaboration with your labs for my future projects..

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December 14, 2021
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December 14, 2021
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December 14, 2021
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March 1, 2022
Abhijit Patil
October 30, 2020

A very good lab delivering acurate results. I must also mention about the very cordial staff that they have.

Raviteja. Vathsavai
December 17, 2021
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April 30, 2022
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December 14, 2021
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December 14, 2021
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April 25, 2022
Samiksha More
June 2, 2022

Great work done by nishka research. Thank you so much for your kind Co operation

Ajay Gunti
December 14, 2021
Sashi Kanth
October 12, 2020

I am a PhD scholar from Kaunas University at Lithuania, Europe. I have given a sample to perform SEM analysis of the organic tissue. The results are highly satisfactory and impressive. The service was really excellent, on time( even before the time schedule), consumer friendly. cost to the academic samples are very less comparing with others. Once again thanks to Nishka for providing me the service ontime as I am really in urgency...

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